Sorting Fluorescent Nanocrystals Using DNA
نویسندگان
چکیده
منابع مشابه
Characterization of Cr(V)-induced genotoxicity using CdTe nanocrystals as fluorescent probes.
CdTe nanocrystals capped by cysteamine were synthesized to study Cr(V)-induced genotoxicity. On the surface of TiO2 thin films, the stepwise process of DNA breakage induced by Cr(V)-GSH complexes was vividly observed by using CdTe-DNA self-assembled fluorescent probes; in acetate buffer solution, an analytical method was developed to detect Cr(V)-induced genotoxicity with CdTe fluorescent probes.
متن کاملFluorescent Contrast agent Based on Graphene Quantum Dots Decorated Mesoporous Silica Nanoparticles for Detecting and Sorting Cancer Cells
Background and Objectives: The inability of classic fluorescence-activated cell sorting to single cancer cell sorting is one of the most significant drawbacks of this method. The sorting of cancer cells in microdroplets significantly influences our ability to analyze cancer cell proteins. Material and Methods: We adapted a developed microfluidic device as a 3D in vitro model to sorted MCF-7 c...
متن کاملIn vivo imaging of quantum dots encapsulated in phospholipid micelles.
Fluorescent semiconductor nanocrystals (quantum dots) have the potential to revolutionize biological imaging, but their use has been limited by difficulties in obtaining nanocrystals that are biocompatible. To address this problem, we encapsulated individual nanocrystals in phospholipid block-copolymer micelles and demonstrated both in vitro and in vivo imaging. When conjugated to DNA, the nano...
متن کاملBi-functional NaLuF4:Gd3+/Yb3+/Er3+ nanocrystals: hydrothermal synthesis, optical and magnetic properties
Magnetic-fluorescent lanthanide doped sodium lutetium fluoride (NaLuF4:Yb3+/Er3+/Gd3+) nanocrystals were synthesized via facile hydrothermal method by varying concentration of Gd3+. Powder X-ray powder diffraction (PXRD), scanning electron microscopy (SEM),transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), p...
متن کاملA dual-fluorescence reporter system for high-throughput clone characterization and selection by cell sorting
Molecular biology critically depends upon the isolation of desired DNA sequences. Flow cytometry, with its capacity to interrogate and sort more than 50,000 cells/s, shows great potential to expedite clone characterization and isolation. Intrinsic heterogeneity of protein expression levels in cells limits the utility of single fluorescent reporters for cell-sorting. Here, we report a novel dual...
متن کامل